internal-organ function, breathing, digestion, and heartbeat. This system consists of two complementary parts: the sympathetic and parasympathetic systems.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
The standard plate count (SPC) method involves diluting 1.0m of bacterial culture into a series of water blanks, and then taking a sample from the water blanks to add to empty petri plates which will be filled with melted agar.
The standard plate count is a method used in microbiology, which is used to gain an insight to estimate the density of bacterial population which is present in a bacterial culture broth. This is done by plating a small concentration of the culture in a petri-dish and then counting the colonies which form in the petri-plate. This method is used mostly in the food industry, to find the density of mesophilic bacteria in food. This method is extremely essential to determine the primary source of the bacterial contaminant.
Learn more about standard plate count here-
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Answer:The ratio of the concentrations of 
and 
when the buffer has a pH of 7.02 is 0.69
Explanation:
The dissociation constant for formic acid = 
Concentration of HA= 0.5 mM
Concentration of = 0.1 mM
pH = 6.16
First we have to calculate the value of 
.
Using Henderson Hesselbach equation :
![pH=pK_a+\log \frac{[A^-}{[HA]}](https://tex.z-dn.net/?f=pH%3DpK_a%2B%5Clog%20%5Cfrac%7B%5BA%5E-%7D%7B%5BHA%5D%7D)
Now put all the given values in this expression, we get:
![6.16=pK_a+\log (\frac{[0.1]}{0.5})](https://tex.z-dn.net/?f=6.16%3DpK_a%2B%5Clog%20%28%5Cfrac%7B%5B0.1%5D%7D%7B0.5%7D%29)
To calculate the ratio of the concentrations of and when the buffer has a pH of 7.02.
Using Henderson Hesselbach equation :
![7.02=6.86+\log \frac{[A^-]}{[HA]}](https://tex.z-dn.net/?f=7.02%3D6.86%2B%5Clog%20%5Cfrac%7B%5BA%5E-%5D%7D%7B%5BHA%5D%7D)
![\frac{[A^-]}{[HA]}=1.44](https://tex.z-dn.net/?f=%5Cfrac%7B%5BA%5E-%5D%7D%7B%5BHA%5D%7D%3D1.44)
![\frac{[HA]}{[A^-]}=0.69](https://tex.z-dn.net/?f=%5Cfrac%7B%5BHA%5D%7D%7B%5BA%5E-%5D%7D%3D0.69)
Thus the ratio of the concentrations of and when the buffer has a pH of 7.02 is 0.69
