The giant axon of a squid, shows that hyperpolarizing stimulus is followed by transient inward capacitive current and lasting outward capacitive current.
Hyperpolarization is a change in a cell's membrane potential that makes it more negative. It is the opposite of a depolarization. It inhibits action potentials by increasing the stimulus required to move the membrane potential to the action potential threshold.
Hyperpolarization can be caused, for instance, by opening channels that allow positive ions to move out of the cell (or negative ions to move in).
The hyperpolarization-activated current is an inward current activated by hyperpolarization from the resting potential and is an important modulator of action potential firing frequency in many excitable cells.
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