A system was developed to measure the efficiency of plasmid gap repair by homologous recombination using either chromosomal or plasmid
<h3>What is
plasmid gap?</h3>
In Saccharomyces cerevisiae, gap repair is a quick and effective technique for putting together recombinant DNA molecules. By homologous recombination between two or more linear DNA fragments, one of which is typically a vector carrying replicative sequences and a selective marker, this technique creates a circular DNA molecule.
Bacteria and certain other microscopic species contain plasmids, which are tiny circular DNA molecules. Physically distinct from chromosomal DNA, plasmids multiply on their own.
In genetic engineering, plasmids are used to amplify, or make several copies of, specific genes. A plasmid is a particular kind of vector used in molecular cloning. A DNA sequence known as a vector is capable of moving foreign genetic material from one cell to another, where the genes can then be expressed and duplicated.
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