The restriction endonuclease EcoRI cleaves the DNA sequence 5'-GAATTC-3' in such a way as to leave a 5' overhang, which is often referred to as a "sticky end". To 5' overhang with the complementary sequence this sticky end adhere.
The palindrome EcoRI restriction site breaks DNA after G and creates sticky ends with AATT. G/corresponding AATTC's DNA sequence is CTTAA/G, where "/" designates the location of the peptide bond break.
When the ssDNA sequence 5'-GAATTC'-3 is recognised by the restriction endonuclease EcoRI, a single-strand cut is made between the G and A nucleotides.
At the ultimate end of double-stranded DNA, an overhang is single-stranded DNA. This can occur on either strand, and if it does, there will either be a 5' overhang on the 5' end of the strand or a 3' overhang on the 3' end of the strand.
Community structure is influenced by many factors, including abiotic factors, species interactions, level of disturbance, and keystone species, play particularly important roles in determining their communities’ structure.
