change in temperature and pH can change the shape of the enzyme and They can break down the reactants
Temperature. Higher temperatures typically result in faster rates of reaction, whether they are enzyme-catalyzed or not. The chemical bonds in the active site, however, can be impacted by temperature changes that are either too high or too low and make them less able to bind substrates. Extremely high temperatures (over 40 degrees Celsius may cause animal enzymes to denature, losing their form and activity)
pH can also affect enzyme function. The acidic or basic characteristics of active site amino acid residues are frequently crucial for catalysis. These residues can be impacted by pH changes, which can make it difficult for substrates to bind. Like with temperature, certain pH ranges (acidic or basic) can cause enzymes to denature. Enzymes function best within these pH ranges.
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