Question

What temperatures are involved in the pcr cycle? what processes take place at each temperature?

Answer 1

The answer to the above question is:

94 degree Celsius for denaturation, 45-65 degree Celsius for primers anneal, and 72 degree Celsius for primer extension.

Denaturation: This process denatures the template (breaks the hydrogen bonds between the two strands) and activates the Taq DNA polymerase. You can adjust the activation period for a particular application or to increase yield, but it is typically kept around 94 degrees C for 2-3 minutes.

The primers anneal to the target sequence region of the template during this stage. The annealing temperature, which is typically 45–65 °C, depends on the Tm of the primers.

Primer extension/elongation: In this stage, Taq polymerase amplifies the target sequence at 72 degrees Celsius for the duration of the target's length, often between 45 and 3 minutes.

What is denaturation?

Denaturation is a biological process that alters a protein's molecular structure. As a result of denaturation, many of the weak connections or bonds (such as hydrogen bonds) that give proteins their highly organized structure in their native (natural) state are broken.

To learn more about denaturation with the help of given link:

brainly.com/question/1303446

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