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Phantasy [73]
1 year ago
10

What temperatures are involved in the pcr cycle? what processes take place at each temperature?

Biology
1 answer:
vlada-n [284]1 year ago
6 0

The answer to the above question is:

94 degree Celsius for denaturation, 45-65 degree Celsius for primers anneal, and 72 degree Celsius for primer extension.

Denaturation: This process denatures the template (breaks the hydrogen bonds between the two strands) and activates the Taq DNA polymerase. You can adjust the activation period for a particular application or to increase yield, but it is typically kept around 94 degrees C for 2-3 minutes.

The primers anneal to the target sequence region of the template during this stage. The annealing temperature, which is typically 45–65 °C, depends on the Tm of the primers.

Primer extension/elongation: In this stage, Taq polymerase amplifies the target sequence at 72 degrees Celsius for the duration of the target's length, often between 45 and 3 minutes.

<h3>What is denaturation?</h3>

Denaturation is a biological process that alters a protein's molecular structure. As a result of denaturation, many of the weak connections or bonds (such as hydrogen bonds) that give proteins their highly organized structure in their native (natural) state are broken.

To learn more about denaturation with the help of given link:

brainly.com/question/1303446

#SPJ4

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the mississippi river carries tons of tiny rock fragments called sediments into the gulf of mexico. what do you think will happe
Monica [59]
The sediments will turn into rock.
5 0
3 years ago
Cross a person who does not have freckles and is heterozygous for dimples with a person who has
Alja [10]

Answer:

1. 1/2

2. 1/2

3. 1/4

Explanation:

Let the allele for freckles be A or a while that of dimples be B or b.

A person without freckles but heterozygous for dimples = aaBb

A person who has freckles (whose mother has no freckles) but no dimples = Aabb

      aaBb    x    Aabb

Offspring

4 AaBb - freckled and dimpled

4 Aabb - freckled and non dimpled

4 aaBb - non freckled and dimpled

4 aabb - non freckled and non dimpled

1. <em>Probability that the parents will have a dimpled child</em> = 8/16 or 1/2

2. <em>Probability that they will have a child with freckles</em> = 8/16 or 1/2

3. <em>Probability that they will have a child with freckles and dimples</em> = 4/16 or 1/4

<u>See the attached image for the Punnet's square analysis of the cross</u>

4 0
3 years ago
A skateboarder has an initial velocity of 3 m/s West and comes to a stop in 2 seconds. What is the skateboarder's
ArbitrLikvidat [17]

Answer:

FV- 0 m/s

IV- 3 m/s

t- 2 sec

A= FV-IV/t

A= 0-3/2

a= -1.5 m/s²

Explanation:

FV- Final Velocity -> m/s

IV- Initial Velocity -> m/s

A- Acceleration -> m/s²

t- time -> seconds

3 0
3 years ago
Read 2 more answers
Would you want to have your DNA profile kept in your medical record as reference for your doctor? Explain your answer
harina [27]

Answer:

yes

Explanation:

6 0
3 years ago
What influences other than humans affect genetic drift in populations?
Lelu [443]

Answer:

Every population experiences genetic drift, but small populations feel its effects more strongly. Genetic drift does not take into account an allele's adaptive value to a population, and it may result in loss of a beneficial allele or fixation (rise to 100% frequency) of a harmful allele in a population.

Explanation:

5 0
3 years ago
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