The answer to the above question is:
94 degree Celsius for denaturation, 45-65 degree Celsius for primers anneal, and 72 degree Celsius for primer extension.
Denaturation: This process denatures the template (breaks the hydrogen bonds between the two strands) and activates the Taq DNA polymerase. You can adjust the activation period for a particular application or to increase yield, but it is typically kept around 94 degrees C for 2-3 minutes.
The primers anneal to the target sequence region of the template during this stage. The annealing temperature, which is typically 45–65 °C, depends on the Tm of the primers.
Primer extension/elongation: In this stage, Taq polymerase amplifies the target sequence at 72 degrees Celsius for the duration of the target's length, often between 45 and 3 minutes.
<h3>What is denaturation?</h3>
Denaturation is a biological process that alters a protein's molecular structure. As a result of denaturation, many of the weak connections or bonds (such as hydrogen bonds) that give proteins their highly organized structure in their native (natural) state are broken.
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