Question

slit2 promotes angiogenic activity via the robo1-vegfr2-erk1/2 pathway in both in vivo and in vitro studies.

Answer 1

Slit2 overexpression promoted angiogenic effects in both a laser-induced CNV mouse model and HUVECs and promoted the biological activity of endothelial cells.

• Recent research has provided novel but contrary insight into the function of Slit2-Robo signaling in angiogenesis.
• Although the role of Robo in choroidal neovascularization (CNV) has been studied, the effect of its ligand, Slit2, on CNV development is unclear.
• Laser-induced CNV in Slit2 transgenic and wild-type mice was used to study the effects of endogenous Slit2 on angiogenesis in vivo.
• Fluorescein angiography was performed to evaluate the leakage area of each lesion.
• Plasmid-based gene transfer technology was used to increase Slit2 expression and to study its effects on human umbilical vein endothelial cells (HUVECs) in vitro.
• Laser treatment led to more CNV and vascular leakage in Slit2 transgenic mice compared with wild-type mice.

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