During prophase the mitotic spindle begins to form.
During prophase, the mitotic spindle starts to self-assemble in the cytoplasm. In contrast, all doubled homologous chromosomal pairs align along the cell's midline, between the two centrioles, during metaphase I. In anaphase, I, spindle fibers connected to the centrioles pull the homologous chromosome pairs apart and to the opposite poles of the cell.
In addition, the microtubules created by the duplicated centrosomes of animal cells are what combine to form the mitotic spindle. Also, chromatin, a combination of DNA and proteins found in the nucleus, condenses during prophase.
The development of discernible chromosomes is caused by the coiling and tightening of the chromatin. One well-organized piece of DNA makes up each chromosome.
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