Substrate -level phosphorylation accounts for approximately 100% of the ATP formed by the reactions of glycolysis.
The direct transfer of a phosphate group to ADP to form ATP is known as substrate-level phosphorylation. It accounts for all of the ATP produced by glycolysis reactions. The first substrate-level phosphorylation occurs when 1,3-diphosphoglyceraldehyde is converted to 1,3 diphosphoglyceric acid and 2 NADH2, resulting in the formation of 6 ATP. Substrate-level phosphorylation converts the 1, 3-diphosphoglyceric acid to 3 phosphoglyceric acid, which produces 2 ATP. The second substrate-level phosphorylation takes place when phosphoenolpyruvic acid is converted to pyruvic acid, which results in the formation of two ATP molecules.
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