Question

explain how dna extraction, pcr, and restriction enzymes were used to get the samples we used for the this gel electrophoresis lab.

Answer 1

Gel electrophoresis is typically used to view (make visible) the outcomes of a PCR process. Using a technique called gel electrophoresis, DNA fragments are separated based on size as they are drawn through a gel matrix by an electric current.

The sample is first heated to cause the DNA to denature, or separate into two pieces of single-stranded DNA, in order to amplify a section of DNA using PCR. Then, using the original strands as templates, an enzyme known as "Taq polymerase" creates two new strands of DNA.

Restriction Gel & Enzyme Digest DNA can be selectively broken into fragments by restriction enzymes and then separated on an agarose gel according to fragment size, as seen by electrophoresis. Since DNA has a negative charge, an electric current.

Learn more about PCR-

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