Answer: Tetracycline.
Explanation:
A plasmid is a small circular DNA molecule found in bacteria that is separate from the bacterial chromosome and replicates independently of it. They encode for certain genes that play a key role in antibiotic resistance. <u>Restriction enzymes are endonucleases that catalyze the cleavage of phosphodiester bonds in different regions located within a DNA strand</u>. PstI is an example of an endonuclease, it is a type II restriction enzyme produced by the microorganism <em>Providencia stuartii</em> that possesses a restriction target in double-stranded DNA dependent on an unmethylated, palindromic, asymmetric sequence, and in this example, it cuts the plasmid at a single site in the ampicillin-resistance gene. After that, the DNA is annealed with another fragment of DNA and this new recombinant molecule is used to transform <em>E. coli</em> cells. Transformation is a key step in DNA cloning because it occurs after restriction enzyme digestion and ligation treatments and transfers newly made plasmids into bacteria. <u>So these bacterias will have a new fragment of DNA, which still has a tetracycline resistance gene but it no longer has the ampicillin resistance gene because it was disrupted by the restiction enzyme</u>. Thereby, the antibiotic resistance phenotype is the tetracycline resistace.
Answer:
Correct
Explanation:
When combining more than two circles in a Venn diagram you have to actually mix in the colors where the intersections are so that you will be able to tell their differences.
Answer:
to continually produce energy,your organs need oxygen and the oxygen is carried in your red blood cells.
Answer:
Frog forelimbs are typically short as the hind limbs are the principal limb pair generating propulsion.
