Answer: A Microtubules
Explanation: The cytoskeleton is composed of at last three different types of fibers: microtubules, microfilaments, and intermediate filaments. These fibers are distinguished by their size with microtubules being the thickest and microfilaments being the thinnest.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
Biology is the study of Living organisms whereby they are clustered into distribution , origin , behavior , anatomy their physiology and morphology.
Biology as a subject has changed my views and conspiracies about the existence of organisms in their ecosystem .Its like from biology you get to know the meaning of life in details right from the origin to death of the organism .
As a human being I have realized much on how biology helps even beyond the class , since I have learnt the reason why each organism belongs to a certain habitat and specific niche of the ecosystem , I have learnt to respect and conserve such organisms as they deserve to exist in that habitat , and their is an interdependency whether direct or indirect .
Also with the knowledge I have acquired on anatomy and the physiology of organisms , I can do a first aid to an individual who has suffered outdoor pass outs or injuries to reduce the impact of the situation before he or she reaches the hospital .
I believe biology has impacted a very important part of my life and I look forward to take further studies in this field to broaden my knowledge even more .
I think the correct answer would be the last option. Monozygotic twins are identical, while dizygotic twins are fraternal. Identical twins involves developing from one zygote where it splits, forming two embryos so that the twins would have more or less the same characteristics. This type of twins can share the same amniotic sac depending on the time the fertilized egg is divided. On the other hand, fraternal twins developed from two eggs wherein they are being fertilized by two different sperm cells resulting to twins which has different characteristics. For this type, the two fertilized eggs would develop separate amniotic sacs.
Answer:
Transcription begins at a gene's promoter, a specific sequence of DNA that acts as a "start" signal for a gene that is to be transcribed. Transcription ends at a sequence of bases that acts as a "stop" signal.
Explanation:
