Characterization of exosomes has become a topic of interest in medical research and molecular biology because it can provide very valuable information for early detection, disease monitoring and development of effective treatments against cancer and autoimmune diseases. Exosomes are known as one type of secreted membrane vesicles that are responsible for intercellular communication by providing means for intercellular exchange of proteins, lipids, mRNA, miRNA and DNA, contributing to intercellular communication in relevant biological processes, playing an important role in the development of several diseases, and specifically, modulating cancer microenvironment and the immune response. Exosomes and its contents also have emerged as non‐invasive biomarkers for various diseases. Exosomes composition and quantity may be directly related to the pathological state of the patient, so the characterization of exosome could be of great relevance for diagnosis.
