Answer: DNA Ligase
Explanation:
During DNA replication, both the parental DNA strands are separated and act as templates for the synthesis of new strands. The formation of the new strands occurs in only 5'-3' direction and thus both the strands are synthesized in a different manner. One new strand with the template of 3'-5' direction will be synthesized continuously and is called a leading strand. The other strand with the template of 5'-3' direction will be synthesized discontinuously in the form of DNA fragments. These fragments are called Okazaki fragments and the strand is called a lagging strand. DNA ligase connects these fragments later to form a continuous lagging strand.
Therefore, DNA ligase is required for ligation of Okazaki fragments, and in absence of DNA ligase, the ligation of fragments will not occur and the newly synthesized strand will be discontinuous. However, if DNA ligase is not present in the reaction mixture, then the chances are that not all the newly synthesized DNA strands will be discontinuous. Only the DNA molecule with the lagging strand will be in the form of fragments.
They cannot reproduce outside of a living cell
Answer:
No
Explanation:
During cyclic photophosphorylation, electrons are cycled continuously out of and back into the reaction center of PSI. It does not involve PSII and plastoquinone. During non cyclic photophosphorylation, the reaction center of plant photosystem II passes electrons to plastoquinone. The reduced plastoquinone carries electrons to the cytochrome b6f complex. Since PSII and plastoquinones are not included in cyclic photophosphorylation, dichlorophenyldimethylurea would not affect the process.
Answer:
They should think of an alternative explanation for their results.
Explanation:
The next step that the scientists should take is to interpret why their hypothesis went wrong. The researchers should look for patterns in their observations and provide an alternative explanation for the results which occurred. Based on the results and their understandings, they should further make another hypothesis and check for its authenticity.
In scientific researches, the results of a hypothesis generally do not get the expected results but this leads to new findings and discoveries.
