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butalik [34]
3 years ago
13

Describe the construction of a recombinant plasmid containing the gene for the green fluorescent protein (GFP) and the insertion

of the plasmid into a bacterial cell by placing the steps in order
-ligate together the GFP gene and the plasmid to generate a recombinant plasmid-transform bacteria with the recombinant plasmid using electroporation

-use PCR to amplify the gene for GFP

-perform a restriction digestion of the GFP gene and the plasmid

-plate the bacterial cells, and screen for positive transformants
Biology
1 answer:
valkas [14]3 years ago
6 0

Answer:

-use PCR to amplify the gene for GFP

-perform a restriction digestion of the GFP gene and the plasmid

-ligate together the GFP gene and the plasmid to generate a recombinant plasmid

-transform bacteria with the recombinant plasmid using electroporation

-plate the bacterial cells, and screen for positive transformants

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