Describe the construction of a recombinant plasmid containing the gene for the green fluorescent protein (GFP) and the insertion
of the plasmid into a bacterial cell by placing the steps in order -ligate together the GFP gene and the plasmid to generate a recombinant plasmid-transform bacteria with the recombinant plasmid using electroporation
-use PCR to amplify the gene for GFP
-perform a restriction digestion of the GFP gene and the plasmid
-plate the bacterial cells, and screen for positive transformants
