Answer:
well..
Explanation:
make sure you use the correct format that you are supposed to and is there a way you could like copy and paste a link? or like the website you used?
The correct answers are the first and second options.
The Compromise of 1850 consisted of five laws passed dealt with the issue of slavery. In 1849, California had requested permission to enter the Union as an antislavery state, potentially upsetting the balance between the antislavery and proslavery states in the U.S. Senate. Senator Henry Clay introduced a series of resolutions seeking a compromise and trying to avert a crisis between North and South. Some of the resolutions were the amnedment of the Fugitive Slave Act and the admission of California to the Union.
The Fugitive Slave Act demands the arrest of all runaway slaves. The Fugitive Slave Act was amended as part of the Compromise of 1850, it allowed for the capture and return of runaway slaves within the territory of the United States; officials who did not arrest an alleged runaway slave were penalized.
California is admitted to the Union as antislavery state. As part of the Compromise, California entered the Union as a free state, with its current boundaries, upsetting the equilibrium that had long prevailed in the Senate.
In order to protect the eggs and developing embryos, frogs produce a thick, sticky coating surrounding the egg called the jelly coat. This jelly coat must be removed before the embryo can be effectively manipulated or microinjected. Jelly coats are important for X. tropicalis development. We have noticed that completely de-jellied embryos often have difficulty gastrulating properly. We believe this is a mechanical problem. The vitelline envelope of X. tropicalis embryos is loose and sticky. Once stuck to the bottom of the dish, it appears to interfere with normal gastrulation.However, simply coating the dishes with a thin layer of agarose prevents this problem. Therefore whenever we completely de-jelly the embryos, we raise them on agarose coated dishes until gastrulation is complete. Once gastrulation is completed, the embryos can be raised in regular petri dishes. If the embryos are not being microinjected, then a partial de-jelly can be very helpful. Often times due to the jelly coat, the embryos can be tightly stuck to each other. A partial de-jelly can separate the embryos and make it easy to transfer from dish to dish. The remaining jelly coat is enough to allow the embryos to gastrulate normally.To de-jelly X. tropicalis embryos, immerse in 3% cysteine made up in 1/9xMR (pH to ~7.5-8.0 with NaOH) or water. Swirl the embryos gently and the jelly coat will slowly be removed. NOTE: Embryos being dejellied before the first cleavage may develop secondary axes if the embryos are swirled too vigorously. Therefore, if the dejellying is being done at the one cell stage, very gentle or no swirling is recommended. For embryos past the first cleavage, we swirl the embryos every so often in an erlenmeyer flask which greatly speeds the process.<span>For a partial de-jelly, immerse in cysteine just long enough until the embryos appear to be separate and sit next to each other in a monolayer. At this point the embryos should be easy to manipulate, and the jelly coat should still be visible around the embryo under the stereomicroscope. For a complete de-jelly, immerse in cysteine until the embryos pack tightly with each other without any clear space between the embryos. Again visualization under the stereomicroscope helps.</span>
When Odysseus set sail away from Calypso's island, Poseidon spotted him and caused him to become shipwrecked. In this way, Poseidon is seen to be a very direct god in that he instantly causes problems for Odysseus himself instead of working through other means like Athena.