Answer:
is bound to the constant region of the secondary antibody.
Explanation:
Enzyme immunoassays are the techniques used to detect the presence of antigens with the help of antibodies. Each of the antibody molecules has a constant and variable region.
The primary antibodies are added to the wells. The constant region of the secondary antibody is bound to an enzyme while its variable region is free so that it can bind to the specific antigen.
Addition of substrate to the system is followed by visualization and/or evaluation of antigen as the reaction between enzyme and substrate produce some visible changes such as color change.
Answer:
b. reducing molecules
Explanation:
Nicotinamide adenine dinucleotide (abbreviated NAD +, and also called diphosphopyridine nucleotide and Coenzyme I), is a coenzyme found in all living cells. The compound is a dinucleotide, as it consists of two nucleotides linked through their phosphate groups with a nucleotide that contains an adenosine ring and the other that contains nicotinamide.
In metabolism, NAD + participates in redox reactions (oxidoreduction), carrying electrons from one reaction to another.
Coenzyme, therefore, is found in two forms in cells: NAD + and NADH. NAD +, which is an oxidizing agent, accepts electrons from other molecules and becomes reduced, forming NADH, which can then be used as a reducing agent to donate electrons. These electron transfer reactions are the main function of NAD +. However, it is also used in other cellular processes, especially as a substrate for enzymes that add or remove chemical groups of proteins, in post-translational modifications. Due to the importance of these functions, the enzymes involved in the metabolism of NAD + are targets for drug discovery.
The basics would be that you'd need to find out if they could exchange genetic information. If not, they couldn't be considered part of one species. Set-up 2 artificial environments so both groups would produce pollen at the same time. Fertilise both plants with the other's pollen. Then fertilise the plants with pollen from their own group.
Count the number of offspring each plant produces.
If the plants which were fertilised by the opposite group produce offspring, they are of the same species. You can then take this further if they are of the same species by analysing if there is any difference between the number (and health) of offspring produced by the crossed progeny and by the pure progeny. You'd have to take into account that some of them would want to grow at different times, so a study of the progeny from their first sprout until death (whilst emulating the seasons in your ideal controlled environment). Their success could then be compared to that of the pure-bred individuals.
Make sure to repeat this a few times, or have a number of plants to make sure your results are accurate.
Or if you couldn't do the controlled environment thing, just keep some pollen one year and use it to fertilise the other group.
I'd also put a hypothesis in there somewhere too.
The independent variable would be the number of plants pollinated. The dependant variable would be the number of progeny (offspring) produced.
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