C. ethnic and religious diversity distinguished the Middle Colonies from New England and the Southern Colonies.
It stemmed from the annexation of the Republic of Texas by the U.S. in 1845 and from a dispute over whether Texas ended at the Nueces River (the Mexican claim) or the Rio Grande (the U.S. claim).
I think the answer is C I remember I took a test for apex and the answer was close to this one. Let me know if I helped!