Answer:
By way of introduction, A260/A280 ratio is use to measure Protein Contamination. This procedure was first described to measure protein purity in the presence of nucleic acids. However it is now commonly used to assess protein contamination of DNA. The method to determine the concentration of protein contamination by using A260/280 ratio method is well explained below by filling in the missing words.
Explanation:
The A260/A280 ratio method estimates protein __purity___ by measuring the absorbance maximum at 280nm caused by the amino acids__cytosine___, ____Adenine____, and ___Guanine_________ Since ___Spectrophotometer____ also absorbs in the UV range, we can correct for this contaminate by measuring the absorbance maximum at ____260nm______ and using the following equation: Concentration (µg/ml) = (A260 reading – A320 reading) × dilution factor × 50µg/ml___ When extrapolated from a standard curve, the Bradford data indicates the amount (in _ug__ ) of __unknown ___ protein found in a sample. If you know the volume of sample that was added to the assay then you can calculate the protein ____concentration ___ of the sample. The coomassie blue dye in the Bradford assay specifically binds primary ¬¬___sulfonic___ and __positive amines__ groups of the amino acid side groups of the proteins. The more the dye binds to the sample, the _anionic_ the blue color will be, and the absorbance at 595¬nm will be_shifted Amax___. A sample with an unusually __protein___ number of ___280nm of Tyrosine __ [give a specific example] amino acids will underestimate the total amount of protein present in the sample.
Answer:
The organisms homeostasis
Answer: d - water forming on the lid under the container
Explanation:
Condensation is the process that describes the change of state of water from gas to liquid. It happens in the water cycle when water vapor gets to the atmosphere and due to the lower temperatures turns into liquid water that then forms clouds.
In the demonstration, the container is meant to be the clouds so when water forms under the container, that is condensation happening because the water vapor has not turned back to liquid after leaving the aquarium water as water vapor.
Denaturation refers to the loss of protein three-dimensional structure when temperature or pH is altered.
Denaturation is the phenomenon in which the tertiary structure of the protein is broken down due to the breakage of bonds between the amino acids of proteins. This breaking of bonds occurs due to the change in pH or temperature from the normal at which the protein remained active.
Three-dimensional structure of the protein is its form in which the protein remains active and functional. It is generally tertiary structure of protein. This active tertiary structure is decided by the sequence of amino acids in the primary structure of protein, where the protein exists as a linear chain.
To know more about denaturation, here
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From what I remember from my bio class, the answer should be True. Hope this helps :)
