The vacuole i believe is the answer
The effect Colchicine might have on dividing cells is that the duplicated chromosomes might not be able to segregate into daughter nuclei.
Colchicine is an alkaloid that attaches to tubulin and prevents it from polymerization to microtubules. As we know that Microtubules are a component of the cytoskeleton of a cell that aids in molecular mobility inside the cell.
During cell division, chromatin condenses into chromosomes, which are then connected to microtubules. The chromosomes are separated into daughter cells by microtubules.
When these cells are treated with colchicine, tubulin polymerization to microtubules may not occur, and the chromosomes containing daughter nucleic might not be separated.
Learn more about chromosomes here:
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<span>The cell has 1% concentration of the salt. The external environment is highly concentrated with 25% saline solution. This will lead to release of water outside the cell, by passive diffusion from a region of high conentration of solvent to lower concentration. Thus, the cell will shrink.</span>
