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lys-0071 [83]
3 years ago
14

Hortense makes up a reaction mix to do Sanger sequencing on a lobster. She mixes the following together: lobster DNA, primers, f

our DNA nucleotides (A, C, G, and T), and DNA polymerase. The reaction does not work. What was the error in Hortense's reaction?
Biology
2 answers:
kifflom [539]3 years ago
6 0
She didn’t cycle the reaction! She has to cycle as follows:
1. 95C-to denature the double stranded DNA
2. 50-55C depends on her primers- to anneal the primers to the single stranded DNA
3. 37-42C depending on the enzyme used - DNA polymerase extends from the primers to make the new DNA.

Also, are the dNTPs special? Either radioactive or with a fluorescence to measure/detect them?
djverab [1.8K]3 years ago
3 0

Answer:

The answer is ( She should have added a small amount of a modified version of one of the four nucleotide.) Just took the test got it right.

Hope this helps:)

Explanation:

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3 years ago
Each of two parents has the genotype green divided by brown​, which consists of the pair of alleles that determine eye color​, a
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A)BB-Bg-Bg-GG

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Explanation:

In order to solve this you just have to create a punnet square, where the parents are shown to both have brown and green coloured eyes genes, so it would be like this:

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Father↓  

Brown                      Brown-Brown                 Brown-Green

Green                      Brown-Green                  Green-Green

As the instructions say that if any kid had one green allele that would be the dominant, only the Brown-Brown will show the fenotype, so there´s 1/4 chance to happen, or 25%, the other probability of having green eyes is 3/4 or 75%

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another example for humans is my parents both hve dark hair but my dad had blone hair when he was younger and so did his mom so i ended up getting a mix of blonde hair with brown! hope this helps!!

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4 years ago
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