The word sponge is more appropriate to use than Underground river since an underground river can loose water while an aquifer cannot loose water, unless it is open-air and above ground. then the aquifer would slowly loose water due to evaporation from the sun.
Answer: If one is constructing a phylogeny of reptiles using DNA sequence data of birds, mammals, amphibians or fish, the suitable outgroup to be used are mammals due to the time of divergence from other group of organisms.
Explanation: Phylogeny is used to determine evolutionary relationship between items or organisms. A phylogenetic tree is a graphical illustration of phylogenetic relationship. In phylogeny, an outgroup represent an organism that is more distantly related to other group of organisms.
In a phylogenetic tree, outgroup stands alone. It shows that the time of divergence of that particular organism is far from other group of organisms. Outgroup is used to root a tree and sometimes represent a group that is more ancestral on a tree.
It should be noted that differences in the DNA sequences of the organisms under consideration will determine which organism will serve as the outgroup.
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No. At that point, you need to determine if the problem lies in the control or the staining reagents or techniques.
