Mutation is the primary cause of all genetic variation. Mutation is important as the first step in evolution, as it produces a new sequence of DNA for a particular gene, producing a new allele. Recombination, through intragenic recombination, may also generate a new DNA sequence (a new allele) for a particular gene.
Klinfelters syndrome is when there is an extra X chromosome in males.
So instead of the normal sex chromosomes for males (XY), a male with klinefelters syndrome will have XXY.
I have included a pic of the karyotype to give you a better idea of what I'm talking about.
Hope this helps! I'm happy to help with any other questions you might have :)
Answer: With the development of powerful microscopes, scientists discovered many single-celled organisms that didn't fit into any of Linnaeus' kingdoms. As a result, a new taxon, called the domain, was added to the classification system.
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When directed mutagenesis which is also called reverse genetics is used, only a single mutation per genome is introduced. In contrast, the number of mutations per genome introduced by random mutagenesis is usually difficult to control. It is likely that the strain you obtained with a mutation in gene X after random mutagenesis contained an additional mutation or perhaps multiple mutations which ultimately was/were responsible for loss of virulence in your favorite pathogen.