Dark-field microscope is a microscope that uses a focused beam of very intense light in the form of a hollow cone concentrated on the sample, where the illuminated object disperses the light and thus becomes visible against the dark background behind it, making the transparent portions of the Specimen remain dark, while surfaces and particles look bright. This form of illumination is used to analyze transparent and unpigmented biological elements, invisible with normal illumination, without killing the specimen.
A light microscope that makes the specimen appear light on a dark background is called a dark field microscope.
Dark field microscopes are light microscopes that are used in different ways to clearly view various specimens that are unstained, transparent, and hard to see using a light field unit. Dark field microscopes are very effective because they show the details of unstained and live samples. It is also very simple to use, and inexpensive to set up.
the process by which different kinds of living organisms are thought to have developed and diversified from earlier forms during the history of the earth.
