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3 years ago

Arrange the following in the proper sequence in which they occur during a single PCR cycle.

Biology

1 answer:

Tju [1.3M]3 years ago

5 0

Answer:

C. 3, 2, 1

Explanation:

During a single PCR cycle, the very first step is the heat separation of the double stranded DNA. Primer binds only single stranded DNA/RNA because it has complimentary bases which match single strand of DNA/RNA that is why it is necessary to unwind both the strands of DNA by providing temperature.

As soon as the DNA strands have separated by increasing the temperature to ~ 95 °C, in the next step the primer binds the portion of DNA which is complimentary to the bases of the primer. In order for primer to bind DNA, the temperature is lowered to ~ 72 °C and the process is called as annealing.

Once primer has bound to the DNA, the next step is polymerization i.e. nucleotides which are the monomer units of DNA are added one by one with the help of enzyme named as Taq polymerase. This is how the strand extends ahead of primer and exact copies of the given DNA are produced.

Note: PCR may be considered as in vitro DNA synthesis or replication.

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What are the differences between motor and sensory pathway? Select all that apply

Mumz [18]

Answer:

sensory path take the impules towards the CNS

motor path take the impules away from CNS

Explanation:

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3 years ago

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When doing medical research with human subjects, which FOUR limitations are unavoidable?

liq [111]

Answer:

Option B, C, D and E

Explanation:

While conducting research on human being, following conditions must be fulfilled

a) The subject must be made aware of the risk and result of the study

b) Subject must be aware of the study objective, time duration and post study impacts

c) Subject must be compensated for any injury caused during the study

d) Informed consent must be taken from the subject

e) The subject must beware of the possible uncontrollable scenarios. Also the test conducted must be testifiable on human being

f) The ill effects of the study can be anticipated if the result obtained are worthy enough

Considering the above facts, option B , C, D and E are unavoidable

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How do mutations enter the gene pool? Why are they important to natural selection?

IRINA_888 [86]

The set of all alleles at all loci is the full gene pool for the species. Over time, the size of a gene pool changes. The gene pool increases when a mutation changes a geneand the mutation survives

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3 years ago

When enzymes in your body digest your food, what type of biomacromolecule is at work?

Lelechka [254]

Answer:

Fat, starch, sugars and protein.

Explanation:

Enzymes break these biomacromolecule bcoz cells get energy by breaking them.

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Within the human body, different cells have different characteristics or phenotypes. For example, neurons look and behave very d

romanna [79]

Answer:

different proteins

Explanation:

All the cells of the body are derived from same cell still different cells have different characteristics or phenotypes. For example, the hemoglobin is present in RBCs only not in any other cell. This is because of a process called gene expression.

This process regulates expression or repression of different set of genes. This makes embryonic cells to become specialized cells that is to perform a specific function. The gene contains DNA which gets transcribed to RNA and then to proteins. The different proteins provides different specificity to cell.

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3 years ago