a. Release of acetylcholine from the synaptic vesicles
Explanation:
The events on neuromuscular junction:
Action potential travels through the membrane of the presynaptic cell causing the voltage-gated channels permeable to calcium ions to open. Ca2+ flow through the presynaptic membrane and increase the Ca concentration in the cell which will activate proteins attached to vesicles that contain a neurotransmitter (e.g. acetylcholine). Vesicles fuse with the membrane of the presynaptic cell, thereby release their contents into the synaptic cleft-space between the membranes of the pre- and postsynaptic cells. Neurotransmitter ACh binds to its receptors on the postsynaptic membrane and its binding causes depolarization of the target cell (muscle cell). Depolarization occurs because sodium enters the cell as a result of neurotransmitter receptor binding.
Law of ecological pyramid - the production of organisms of each subsequent trophic level is always on average up to 10 times less than the production of the previous one
I think the correct answer from the choices listed above is option A. Directional selection can be distinguished from disruptive selection by the elimination of extreme variations of traits. Directional selection is a natural selection where an extreme phenotype is favored than others.