Answer:
It was the first armed war.
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One reason conquistadors failed to establish colonies is that the Natives kicked them out. Many of the aboriginal people were very powerful and could easily get rid of the conquistadors.
Conquistadors could also run out of resources. Nothing lasts forever, and if they couldn't obtain enough resources while exploring and settling it would be hard to establish a colony easily.
There could also be dangerous things that could hinder the settlements such as wild beasts, natural events, and diseases.
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The completion of the Transcontinental Railroad greatly affected companies that made products in that it made it far easier for them to transport their goods over long distances to markets, which greatly increased profits and output.