This is because there might not be one restriction site bordering the gene to be cloned and the identical restriction site in the plasmid. Since these two restriction enzymes make compatible sticky ends, the insert has a chance of combining with the plasmid. To ensure efficient digestion, the two recognition sites should be more than 10 base pairs apart. If one of the enzymes is a poor cutter or if the sites are separated 10 base pairs or less, the digestions <span>should be performed sequentially</span>
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Two types of relationships between variables are direct and inverse variation. ... As one variable increases, the other also increases, and as one decreases, the other also decreases. In contrast, inverse variation suggests that variables change in opposite directions. As one increases, the other decreases and vice versa.
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Cell theory is a scientific theory first formulated in the mid-nineteenth century, that living organisms are made up of cells, that they are the basic structural/organizational unit of all organisms, and that all cells come from pre-existing cells
ex. Any animal
42-rationale: each unit of packed rbcs contains 250 ml. each unit is to infuse over 1 hour (60 minutes).\r\nuse the following equation:\r\n250 ml/60 minutes = 4.16 ml.\r\nmultiply by the drip factor:\r\n4.16 ml 10 gtt = 41.6 gtt/minute (42
