WORKING DISTANCE is space between the bottom of objective the lens and the surface of the slide.
The distance between front edge of the objective and the lens and the specimen surface ("with the surface of the cover glass in case of the cover glass objective lens") when the specimen is also focused.
The working distance (W.D.) which is determined by the linear measurement of the objective and front lens to the focal plane. In the general, the objective of the working distance decreases by the magnification and the numerical aperture of both increase. The working distance is the distance and from the front of the lens to be the focal plane
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Answer:
Explanation:
The cell membrane separates the cell from the outer environment. The extracellular fluid contains the sodium ions (Na+), chloride ions (Cl-), while intracellular fluid contains potassium (K +) and negative anions.
The potential difference arises when the membrane is selectively permeable to some ions. The resting potential is -70mV.
When the neurons get excited, the sodium ions start to enter by sodium channels.
Now there are more positive ions inside the cell membrane. It disturbs the resting potential i.e. -70mV. This stage is known as depolarization.
When the inside environment of the cell is more positively charged, the potassium ions start to move out of the cell. It goes out by the voltage-gated channels. Thus resting stage is maintained and it is known as repolarization.
But the initial stability of the cell membrane has to be maintained. To restore the resting stage, the sodium ions start to move out of the membrane and potassium ions enter into the cells again. This is an active transport and has done by the Na+ - K+ pump. Here 3 sodium ions move out and 2 potassium ions pumped into the cell through the plasma membrane.
Thus the resting potential regains. The potassium ions come back into the cells against the concentration gradient and ATP provides the energy for this phenomena.
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Where the picture but,The Plasma membrane maintains the homeostasis throughout a cell because it <span>filters what substances go in and out of the cell. </span>