Answer: DNA Ligase
Explanation:
During DNA replication, both the parental DNA strands are separated and act as templates for the synthesis of new strands. The formation of the new strands occurs in only 5'-3' direction and thus both the strands are synthesized in a different manner. One new strand with the template of 3'-5' direction will be synthesized continuously and is called a leading strand. The other strand with the template of 5'-3' direction will be synthesized discontinuously in the form of DNA fragments. These fragments are called Okazaki fragments and the strand is called a lagging strand. DNA ligase connects these fragments later to form a continuous lagging strand.
Therefore, DNA ligase is required for ligation of Okazaki fragments, and in absence of DNA ligase, the ligation of fragments will not occur and the newly synthesized strand will be discontinuous. However, if DNA ligase is not present in the reaction mixture, then the chances are that not all the newly synthesized DNA strands will be discontinuous. Only the DNA molecule with the lagging strand will be in the form of fragments.
Answer:
Trichomoniasis
Explanation:
Trichomoniasis is an sexually transmitted disease that affects around 30% of world population. The common symptoms are frequent urge to urinate, vaginal bleeding, burning sensation during urination.
The causative agent of trichomoniasis is a protozoa called <em>Trichomonas vaginalis</em>. This protozoan can be transfer through the genital contact. The protozoa can easily be transfer from affected individual to the healthy individual.
Thus, the correct answer is option (3).