In the sanger (dideoxy) method for dna sequencing, a small amount of a dideoxynucle- otide triphosphate—say, ddctp—is added to t

Question

3 years ago

10

In the sanger (dideoxy) method for dna sequencing, a small amount of a dideoxynucle- otide triphosphate—say, ddctp—is added to t

he sequencing reaction along with a larger amount of the corresponding dctp. what result would be observed if the dctp were omitted?

Biology

2 answers:

Ugo [173] · Answer 1 · 2022-03-19T15:18:58+03:00

Ugo [173]3 years ago

7 0

When the first G residue is encounteredin the template, ddCTP is added and polymerization halts. Only one band will appear in the<span>sequencing gel.

Hope this helps !

Photon</span>

n200080 [17] · Answer 2 · 2022-03-19T17:38:36+03:00

n200080 [17]3 years ago

5 0

I agree with photon, if you have any trouble, maybe you can visit the site below.

https://www.cd-genomics.com/complete-plasmid-dna-sequencing.html