In the sanger (dideoxy) method for dna sequencing, a small amount of a dideoxynucle- otide triphosphate—say, ddctp—is added to t he sequencing reaction along with a larger amount of the corresponding dctp. what result would be observed if the dctp were omitted?
2 answers:
When the first G residue is encounteredin the template, ddCTP is added and polymerization halts. Only one band will appear in the<span>sequencing gel. Hope this helps ! Photon</span>
I agree with photon, if you have any trouble, maybe you can visit the site below.
https://www.cd-genomics.com/complete-plasmid-dna-sequencing.html
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