Yes. In the beginning, glycolysis requires 2 ATP molecules.
One ATP allows for the phosphorylation of the glucose molecule to fructose-6-phosphate and another phosphorylation to fructose-1,
6-biphosphate. The fructose-1, 6- bisphosphate
is then cleaved into two glyceraldehyde-3-phosphates that then enter the energy
generating part of the glycolysis biochemical reaction.
