The DNA region is easily accessible
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The answer is interconnected. You will come to find that everything that makes life possible on earth is all interconnected. It is just like the way all the human activities and necessities for the everyday life are all interconnected to nature.
Answer:
The archaea and the bacteria both are prokaryotes. However, the genetic makeup of the archaea is more similar to the eukaryotes. Moreover, they have differences, in their metabolic pathways, genes and the enzymes possessed by them.
Explanation:
The differences between Archaea and bacteria:
1. The cell wall of the bacteria consist of peptidoglycan, while the cell wall of the archaea consist of pseudo-peptidoglycan.
2. The bacteria are capable of spore formation, which can lie dormant for long periods of time until a suitable condition is found for their growth. The archaea are not known to form such spores.
3. The genes of the archaea are more similar to the eukaryotes than the bacteria.
4. The bacteria are found everywhere where the living conditions are suitable (soil, air, living beings, non-living things). the archaea are capable of surviving in extreme conditions (hot springs, salt brine).
5. The bacteria use the process of glycolysis and follows Kreb's cycle for glucose break-down. The archaea do not undergo glycolysis or Kreb's cycle.
The restriction endonuclease EcoRI cleaves the DNA sequence 5'-GAATTC-3' in such a way as to leave a 5' overhang, which is often referred to as a "sticky end". To 5' overhang with the complementary sequence this sticky end adhere.
- The palindrome EcoRI restriction site breaks DNA after G and creates sticky ends with AATT. G/corresponding AATTC's DNA sequence is CTTAA/G, where "/" designates the location of the peptide bond break.
- When the ssDNA sequence 5'-GAATTC'-3 is recognised by the restriction endonuclease EcoRI, a single-strand cut is made between the G and A nucleotides.
- At the ultimate end of double-stranded DNA, an overhang is single-stranded DNA. This can occur on either strand, and if it does, there will either be a 5' overhang on the 5' end of the strand or a 3' overhang on the 3' end of the strand.
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