In Tennessee William's play A Streetcar Named Desire, Blanche tells her sister those words and the theme of the play that those lines reflect are "a woman's dependence on men."
The quote from chapter 22 of The Adventures of Huckleberry Finn that contains sarcasm is "Ladies and Children Not Admitted" saying that if such line does not ring a bell and bring audience's attention then they don't know Arkansas at all.
The theme here is that his daughter has changed because of her friends. She became closer to them than her father and spends more time with them than him. The narrator wants to correct this that is why he wants to talk to her.
You, you said the word " I ", so you are the first subject.