Answer:
Denaturation process: The DNA template
Annealing process: Primers
Elongation process: dNTPs and Taq polymerase
Explanation:
For the denaturing process, the only ingredient that is required is the DNA template that will be separated from a double helix (or double strand) into a single strand, by increasing the temperature to 95 C, (at this temperature the hydrogen bonds that keep together the double stranded break). After the double strand is denatured, the following process is annealing. For this, the required ingredient are the primers; these primers will hybridize or anneal according to the nucleotide complementarity to the single strand of the DNA. Finally, for the Elongation process, you will require the Taq polymerase and the dNTPs. The enzyme will synthesize or “generate” a new strand of DNA based on the DNA template, using the provided dNTPs in the direction 5’ to 3’.
I hope this clarify you inquiry.
The answer is B)
The Golgi apparatus is located in the cells. It is an extra combination of organelle similar to the ER. This was named following Camillo Golgi.
(Notice that his last name is Golgi)
Answer:
Laws of stratigraphy help scientists determine the relative ages of rocks. This law states that deeper rock layers are older than layers closer to the surface. An unconformity is a gap in rock layers. They occur where older rock layers eroded completely before new rock layers were deposited.
Explanation:
The study of rock strata is called stratigraphy. The laws of stratigraphy can help scientists understand Earth’s past. The laws of stratigraphy are usually credited to a geologist from Denmark named Nicolas Steno. He lived in the 1600s.
Hope this helps!
Brain-List?
Answer:
No
Explanation:
Freshwater biology is the scientific biological study of freshwater ecosystems and is a branch of limnology.
Answer:
The correct answer is : A.
Explanation:
- This is because of the following reasons:
- Escherichia coli is a bacteria and hence is a prokaryote.
- The cloning of human (eukaryote) genes is done to obtain in-vitro protein expression which are to be used a pharmaceutical compounds.
- Although the phenomenon of Central Dogma takes place in both Prokaryotes and Eukaryotes, the mechanism is very different.
- Some of the differences includes:
- Eukaryotic genes contain certain non-protein encoding sequences called the Intron sequences which needs to be removed from the transcribed mature mRNA by Spliceosome. This phenomenon is absent in prokaryotes as they do not possess introns. Hence, proper processing of eukaryotic mRNA is not possible in prokaryotes.
- The mature eukaryotic mRNA has a 5' cap and 3' polyadenylated tail which is added by a capping enzyme and a poly-A polymerase enzyme to increase their stability. This will not be possible in a prokaryote as they lack these enzymes.
- The translation of membrane proteins and secretory proteins is carried on by the ribosomes bound to the endoplasmic reticulum. This structure is absent in prokaryotes, hence they will be unable to produce a proper polypeptide sequence.
- Finally, the eukaryotic proteins undergo various modifications after formation, like methylation, acetylation, etc. These reactions cannot be carried out in a prokaryote as they lack the respective enzymes.
- Hence, we see a properly folded functional eukaryotic protein cannot be produced in a prokaryote.
