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3 years ago

Rank the steps of the (sandwich) ELISA procedure from first step to last step. Do not overlap any steps.

Business

1 answer:

Anvisha [2.4K]3 years ago

8 0

Answer and Explanation:

The ELISA refers to the enzyme-linked immunosorbent assay (ELISA) It is used to determine the existence of an antigen in a sample with the help of antibiotics

The ELISA procedure in sequence form is shown below:

1. The capture antibody is added and then clean it

2. Now adding the blocking buffer and then clean it

3. Now add the samples with controls, Hatch it and clean it

4. Add horseradish peroxidase (HRP) conjugated with the antibody, Hatch it and clean it

5. Add Thymidine monophosphate (TMP)

6. And finally, the last step is to record the results

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Suppose Charlene Brewster has timesâ (in seconds) of 8.5â, 8.5â, 8.3â, 8.6â, 8.7â, 8.4 and a performance rating of 85â%.

professor190 [17]

Answer:

1. 10s

2. Slower than normal

Explanation:

1. To calculate the normal time, we first take the average of Charlene's observed times:

Average of Charlene's observed time = $\frac{(8.5+8.5+8.3+8.6+8.7+8.4)}{6}$ = 8.5s

Her normal time is therefore: $\frac{Average of Charlene's observed time}{performance rating}$ = $\frac{8.5}{0.85}$ = 10s

2. Since no of Charlene's observed time is higher than normal time of 10s, we can therefore conclude that her work perfomance should be rated as slower than normal.

7 0

3 years ago

True or false: in situations where an annual budget deficit exists, cutting expenses from the budget is optimal.

My name is Ann [436]

The statement in situations where an annual budget deficit exists, cutting expenses from the budget is optimal is True.

<h3>What is budget deficit?</h3>

Budget deficit tend to occur when the expenses or expenditure is higher then the revenue.

Cutting down expenses from the budget is most desirable if we want to have budget surplus. Budget surplus is when revenue is higher than expenditure.

Therefore the statement in situations where an annual budget deficit exists, cutting expenses from the budget is optimal is True.

Learn more about budget deficit here:brainly.com/question/26010226

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5 0

1 year ago

A truck acquired at a cost of $69,000 has an estimated residual value of $12,000, has an estimated useful life of 300,000 miles,

Anuta_ua [19.1K]

Answer:

A. $57,000

B. Depreciation rate per mile is $0.19

C. Depreciation is $14,630

Explanation:

a. cost of the truck less the residual value.

Cost of the truck $69,000

Less: Residual value <u>$12,000</u>

$57,000

b. Depreciation rate per mile is computed by dividing cost of the truck less the residual value over the estimated useful life.

$57,000 / 300,000 miles = $0.19

c. Units-of-activity depreciation for the year is computed by multiplying miles driven for the year by depreciation rate per mile.

77,000 miles x $0.19 = $14,630

6 0

3 years ago

g On June 30, the value of the account is 157.50. On December 31, the value of the account is X. Using the time weighted method,

irga5000 [103]

Answer:

236.23

Explanation:

The computation of X is shown below:-

As per the time-weighted method

The 6-month yield

= (40 ÷ 50) × (80 ÷ 60) × (157.50 ÷ 160) - 1

= 5%

Annual equivalent = (1.05)^2 - 1

= 10.25%

1 - year yield = (40 ÷ 50) × (80 ÷ 60) × (175 ÷ 160) × (X ÷ 250) - 1

= 0.1025

X(0.004667) = 1.1025

X = 236.23

Therefore on December 31st the value of account of X = 236.25

4 0

3 years ago

Kalyan Singhal Corp. makes three products, and it has three machines available as resources as given in the following LP problem

alukav5142 [94]

Answer:

a) X1=5.64

X2=4.57

X3= 8.59

Optimal solution=104.77

b) Additional hour on machine 3 = 105.42 – 104.77 = 0.64

c) Additional 15 hours on machine 2 = 105.37-104.77 = 0.6

Explanation:

Please consider the data provided by the exercise. If you have any question please write me back. All the exercises are solved in a single sheet with the formulas indications. SOLVED WITH EXCEL SOLVER.

4 0

3 years ago