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Sergio039 [100]
3 years ago
11

Rank the steps of the (sandwich) ELISA procedure from first step to last step. Do not overlap any steps.

Business
1 answer:
Anvisha [2.4K]3 years ago
8 0

Answer and Explanation:

The ELISA refers to the enzyme-linked immunosorbent assay (ELISA) It is used to determine the existence of an antigen in a sample with the help of antibiotics

The ELISA procedure in sequence form is shown below:

1. The capture antibody is added and then clean it

2. Now adding the blocking buffer and then clean it

3. Now add the samples with controls, Hatch it and clean it

4. Add horseradish peroxidase (HRP) conjugated with the antibody, Hatch it and clean it

5. Add Thymidine monophosphate (TMP)

6. And finally, the last step is to record the results

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Airborne Airlines Inc. has a $1,000 par value bond outstanding with 10 years to maturity. The bond carries an annual interest pa
yanalaym [24]

Answer:Yield to maturity is 9.59%;  After tax cost of debt =7.672%

Explanation:

 A)   Yield to maturity ={ C + (FV-PV)/t} /  {(FV +PV)/2}

Where C – Interest payment    = $90

FV – Face value of the security

= $1000

PV – Present value/curent market value = $960

t – years it takes the security to reach maturity= 10 years

imputing the values and calculating,

yield to maturity ={ C + (FV-PV)/t} /  {(FV +PV)/2}

= $90 + (1000-960)/10} / 1000 + 960 /2

$90 + 4= $94 /980= 0.0959

therefore Yield to maturity is 9.59%

B)   After tax cost of debt =    Yield To Maturity  x (1 - tax rate)

=9.59% x (1-20%)= 9.59% x (1-0.2 )= 9.59% x 0.8 =

9.59 % x 80%=7.672%

4 0
3 years ago
Which one of the following types of charitable contributions is NOT deductible for federal income tax purposes?
Fofino [41]

Answer: B.

Explanation: Contributions to individuals, foreign governments, foreign charities, and certain private foundations similarly are not deductible.

3 0
3 years ago
Consider a city of 200 people (100 rich and 100 poor) and two neighborhoods (100 people in each). Both groups generally prefer t
Mekhanik [1.2K]

Answer:

Explanation:

Step 1. Given information.

  • City of 200 people
  • 100 rich, 100 poor.

Step 2. Formulas needed to solve the exercise.

  • P(poor) = 0.9x^2
  • P(rich)= 35x-0.1x^2

Step 3. Calculation and step 4. Solution.

P(poor) = p (rich)

0.9x2 = 35x - 0.1x2

1x2 = 35x

x = 35

x is the percentage of rich above 50%, thus there are 35% rich people above 50%.

P (poor) = 1102.5

P (rich) = 1102.5

The equilibrium premium is $1,102.5

3 0
4 years ago
a report made to the U.S. Congress in 2001, the National Academy of Sciences cautioned that if fuel economy encourages the produ
kakasveta [241]

Answer:

there is a tradeoff between safety and fuel economy

Explanation:

Tradeoff is also known as opportunity cost. It is the cost of the next best option forgone when one alternative is chosen over other alternatives

By choosing fuel economy, auto manufacturers are reducing safety of cars. This is an example of a tradeoff

6 0
3 years ago
Dakota Company experienced the following events during Year 2. Acquired $30,000 cash from the issue of common stock. Paid $12,00
a_sh-v [17]

Question Completion:

January 1 general ledger balances: Cash = $2,000, Land $12,000, Notes Payable $0, Common Stock $6,000, and Retained $8,000.

Answer:

Dakota Company

Event      Assets           = Liabilities   + Stockholders Equity    Account Titles

              Cash   Land   = Accts Payable Common  Retained    for Retained

                                                                  Stock      Earnings      Earnings

Balance 2,000 12,000 = 0                +   6,000          8,000

1.          30,000             =                    + 30,000

2.        -10,000 +10,000

3.         10,000              =  10,000

4.        20,000              =                                          20,000 Service Revenue

5.          -1,000              =                                           -1,000 Utilities Expense

6.       -15,000               =                                        -15,000 Operating Exp.

7.        -2,000               =                                         -2,000

8.                           700 =                          +700

Bal. $34,000 $22,700 = $10,000   + $36,700     $10,000

b-1. Income Statement for the year ended December 2018:

Service Revenue      $20,000

Operating expenses   15,000

Utilities expense           1,000

Total expenses        $16,000

Net Income               $4,000

b-2. Statement of changes in equity for the year ended December 31, 2018:

Common stock, January 1    $6,000

Additional common stock    30,000

Land Revaluation                       700

Common stock, Dec. 31     $36,700

Retained earnings,

January 1                   8,000

Net Income                4,000

Dividends                 -2,000

Retained earnings             $10,000

Total equity                       $46,700

b-3. Balance Sheet as of December 31, 2018:

Assets:

Cash                                      $34,000

Land                                        22,700

Total assets                         $56,700

Liabilities and Equity:

Liabilities                              $10,000

Common stock                     36,700

Retained earnings                10,000

Total liabilities and equity $56,700

c. Percentage of assets provided by retained earnings

= $10,000/$56,700 * 100 = 17.64%

Yes.  The cash in retained earnings = $34,000 * 17.64% = $5,998.

Explanation:

a) Data and Calculations:

Analysis of Transactions during Year 2:

Cash $30,000 Common Stock $30,000

Land $12,000 Cash $12,000

Cash $10,000 Loan $10,000

Cash $20,000 Service Revenue $20,000

Utilities Expense $1,000 Cash $1,000

Operating Expenses $15,000 Cash $15,000

Dividends $2,000 Cash $2,000

Land $700 Revaluation $700

5 0
3 years ago
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