Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
In the United States the direction the continental polar air mass move South toward the Gulf of Mexico to replace the rising maritime tropical air mass.
Answer: Option A
<u>Explanation:</u>
Air masses are large bodies of air moving horizontally in a very uniform way. Continental polar air mass is an air mass that forms near the poles. It originates in northern Canada and Alaska and move south then East.
The continental polar masses are the causes for the severe cold weather in the South during the cold months. Maritime tropical mass are warm and have high moisture content. During winter it comes in contact with the continental polar mass hence getting stabilized.
Mitosis is the process of cell division, where one parent cell divides to produce two genetically identical daughter cells. This process is vital in growth and tissue repair.
The reason that tissue from the fetal stage is helpful in studying mitosis is because mitosis is continuously and rapidly occurring in this phase of life in humans. The high rate of mitosis is due to the need for the fetus to grow rapidly and develop the necessary parts for it to be born.
Explanation:
Fats are very important classes of food needed by the body. Excess fat is not good for the body.
The following are the reasons fats are essential for the body;
- They provide energy for the body too.
- Fats are good insulators helping to keep the body warm.
- They help the body absorb vitamins because some vitamins are only soluble in fats.
- They help in growing of the cell.
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