On the off chance that there is an EcoR1 site in either the center of the Glo quality, or amidst the selectable marker site in the plasmid, it would likely debilitate either Glo, or the plasmid.I hope this will help.
G1 is followed by S phase (synthesis), during which DNA replication takes place. The completion of DNA synthesis is followed by the G2 phase (gap 2), during which cell growth continues and proteins are synthesized in preparation for mitosis.
