Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
I think it would be Ionic Solution
The best answer is C
Hope it helped!! :)
Clouds and Strom
The troposphere is the most minimal layer of Earth's environment that is closest to humans , and is likewise where almost all climate conditions happen. It contains roughly 75% of the climate's mass and 99% of the aggregate mass of water vapor and pressurized weather elements occur
Answer:
I think s its True
Explanation:
What do low levels mean? Low TSH levels indicate hyperthyroidism. This is also known as an overactive thyroid. If the thyroid gland is secreting levels of hormones that are too high, the pituitary gland produces less TSH
hope it helps