Question

During pyrosequencing, how are the nucleotides that bind to template DNA identified?

Answer 1

Answer:

The correct answer is option e. "pyrophosphate is converted to ATP so that luciferase is activated".

Explanation:

Pyrosequencing is a method of DNA sequencing that allows to detect the nucleotides that are being incorporated to a DNA template mediated by a polymerase. In the reaction mixture, pyrophosphate (PPi) is released as a result of nucleotide incorporation by polymerase. As pyrophosphate is released, it is converted to ATP so that luciferase is activated by the oxidation of luciferin. Luciferase is the enzyme that mediates the reaction that generates light and allows to identify the nucleotides that are being incorporated.