Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
The answer is <em>an</em><em> </em><em>incorrect</em><em> </em><em>hypothesis</em><em> </em><em>means</em><em> </em><em>the</em><em> </em><em>experiment</em><em> </em><em>fail</em><em>.</em><em>.</em><em>.</em><em> </em>
<h2>
<em>WHY</em></h2>
<em>Hypothesis</em><em> </em><em>is</em><em> </em><em>a</em><em> </em><em>tentative</em><em> </em><em>statement</em><em> </em><em>that</em><em> </em><em>relates</em><em> </em><em>the</em><em> </em><em>manipulated</em><em> </em><em>variable</em><em> </em><em>ro</em><em> </em><em>to the</em><em> </em><em>responding</em><em> </em><em>variable</em><em> </em>
(which may be true or false)
<em>Hope</em><em> </em><em>this is</em><em> </em><em>correct and</em><em> </em><em>helpful</em><em> </em>
HAVE A GOOD DAY!
Answer:
In cells, some molecules can move down their concentration gradients by crossing the lipid portion of the membrane directly, while others must pass through membrane proteins in a process called facilitated diffusion.
Explanation:
<h2>Follow instagrm at --> mvnnyvibes</h2>
Answer:
Nucleus
Explanation:
Prokaryotes are the organism that came earlier than eukaryotes on this earth. These prokaryotes do not have a nucleus and other membrane-bound organelles like chloroplast, mitochondria, Golgi apparatus, endoplasmic reticulum, etc.
So as prokaryotes lack membrane-bound organelle their genetic material is not enclosed in the nucleus therefore their genetic material remains in the cytoplasm of the cell while eukaryotes have membrane-bound nucleus in which their genetic material are present.
So the nucleus is the major organelle that prokaryotes are missing that eukaryotes have.