Answer:
Staphylococcus aureus, Pseudomonas aeruginosa, and E. coli
Explanation:...
Answer: Not totally certain if you have any multiple choice answers or anything but here's a general idea of what I would write in response.
Explanation: First step is always going to be asking them if they're okay, the question says they're unresponsive so you want to access the situation. If they are spasming and signs lead to them having a seizure your going to immediately call for an ambulance, don't try to restrain them unless they are potentially going to hurt themselves ie. falling off of something or hitting something dangerous nearby. Restraining them can potentially damage their nerves and muscles more than just letting them flail.
If they don't seem to be having any signs of a seizure then you want to check if they're breathing. If they aren't you need to start performing cpr. Have someone nearby call an ambulance or call 911 and ask for walk-through steps if you aren't certified, that way you know what your doing and you can have that support you if they try to sue you (which won't be a problem because of the good samaritan law anyways) You want to get them laying on their back, tilt their head and make sure nothings restricting their air flow. Then your going to do a couple breaths, if they don't start breathing you'll begin compressions. Compressions, breath, compressions breath. I reccommend looking up appropriate compression techniques for different age people and how to correctly administer if you aren't already aware since I don't want to write a whole article here- I hope this helped!
Answer:
Okay
Explanation:
Human topoisomerase I plays an important role in removing positive DNA supercoils that accumulate ahead of replication forks. It also is the target for camptothecin-based anticancer drugs that act by increasing levels of topoisomerase I-mediated DNA scission. Evidence suggests that cleavage events most likely to generate permanent genomic damage are those that occur ahead of DNA tracking systems. Therefore, it is important to characterize the ability of topoisomerase I to cleave positively supercoiled DNA. Results confirm that the human enzyme maintains higher levels of cleavage with positively as opposed to negatively supercoiled substrates in the absence or presence of anticancer drugs. Enhanced drug efficacy on positively supercoiled DNA is due primarily to an increase in baseline levels of cleavage. Sites of topoisomerase I-mediated DNA cleavage do not appear to be affected by supercoil geometry. However, rates of ligation are slower with positively supercoiled substrates. Finally, intercalators enhance topoisomerase I-mediated cleavage of negatively supercoiled substrates but not positively supercoiled or linear DNA. We suggest that these compounds act by altering the perceived topological state of the double helix, making underwound DNA appear to be overwound to the enzyme, and propose that these compounds be referred to as ‘topological poisons of topoisomerase I’
Answer: Hold the microscope with one hand around the arm of the device, and the other hand under the base. This is the most secure way to hold and walk with the microscope. Avoid touching the lenses of the microscope.
