The plasmid must express a gene for ampicillin resistance (the protein product of the bla gene codes for beta-lactamase, the protein that breaks down ampicillin). The colonies on the ampicillin plate are antibiotic resistant. This means that they have taken up the transformed plasmids expressing both the bla gene and the GFP gene.

Explanation:

The transformation involved the genetic modification of a plasmid to incorporate the gene encoding the green fluorescent protein (GFP) from jelly fish. GFP makes cells glow under UV light.

In genetic engineering, scientists use antibiotic resistance as markers to indicate cells that have been transformed. By incorporating an antibiotic resistance gene such as bla into the vector (plasmid) and then growing the cells in antibiotic media, scientists determine which colonies have taken up the plasmid. Therefore, if the cells survive, this means that they contain the plasmid with antibiotic resistance gene as well as the GFP gene.

5 0

3 years ago

Which food group is the best source of protein from the food pyramid please help

34kurt

Answer:

Meat is a major source of protein, as well as iron, zinc, and vitamin B12. Meats, poultry, and fish include beef, chicken, pork, salmon, tuna, shrimp, and eggs. The meat group is one of the major compacted food groups in the food guide pyramid.

Explanation:

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6 0

2 years ago

Geologists, more specifically mineralogists, identify minerals based on ________. A. Optical properties. B. Solid properties. C.

Shkiper50 [21]

The minerals are mostly characterized by the Physical properties (D) which includes the hardness, lustre, density etc. The mineralogists check the detailed view of minerals by using high powered microscope.

The minerals are chemical compounds which are formed as a result of physical processes or geological processes. They mostly occur in crystalline form. For Example: Cacite, Sulfur, Quartz etc $.$

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5 0

3 years ago

Suppose that a biologist states that the average height of undergraduate students at your university is 205 cm plus or minus a s

GrogVix [38]

Answer:

This means that the means height (which is all the sum of the height of all the students divided by their number ) is 205 cm. Standard deviation means how the height of the students deviates from this mean since students do vary in height and are not all the same height of 205 cm. Therefore, in this case, the amount of standard dispersion of the students' height is by 17 cm on either lower or higher side of the mean.

7 0

3 years ago