$3.073 billion and<span> $642.7 million</span>
Sorry can’t help u plz give me thanks and brainliest
This would be the atomic mass. Hope this helps!! :)
Answer:
D. The last option
Explanation:
You can't divide by 0, so find out when the given function is discontinuous.
You can see that x is discontinuous at x=-1,1, so find a piecewise function that fill in those gaps.
Plug x=-1,1 into your function to find out what values you need to fill in. But of course it's discontinuous at those points, so plug in a number near x=1 and x=-1. I chose x=-1.1 and x=1.1
so we need to fill in 2 at x=-1, and fill in 4 at x=1
Answer:Objective: To determine the combined effect of oxygen level and glucose concentration on cell viability, ATP production, and matrix synthesis of temporomandibular joint (TMJ) disc cells.
Design: TMJ disc cells were isolated from pigs aged 6-8 months and cultured in a monolayer. Cell cultures were preconditioned for 48 h with 0, 1.5, 5, or 25 mM glucose DMEM under 1%, 5%, 10%, or 21% O2 level, respectively. The cell viability was measured using the WST-1 assay. ATP production was determined using the Luciferin-Luciferase assay. Collagen and proteoglycan synthesis were determined by measuring the incorporation of [2, 3-(3)H] proline and [(35)S] sulfate into the cells, respectively.
Results: TMJ disc cell viability significantly decreased (P < 0.0001) without glucose. With glucose present, decreased oxygen levels significantly increased viability (P < 0.0001), while a decrease in glucose concentration significantly decreased viability (P < 0.0001). With glucose present, decreasing oxygen levels significantly reduced ATP production (P < 0.0001) and matrix synthesis (P < 0.0001). A decreased glucose concentration significantly decreased collagen synthesis (P < 0.0001). The interaction between glucose and oxygen was significant in regards to cell viability (P < 0.0001), ATP production (P = 0.00015), and collagen (P = 0.0002) and proteoglycan synthesis (P < 0.0001).
Conclusions: Although both glucose and oxygen are important, glucose is the limiting nutrient for TMJ disc cell survival. At low oxygen levels, the production of ATP, collagen, and proteoglycan are severely inhibited. These results suggest that steeper nutrient gradients may exist in the TMJ disc and it may be vulnerable to pathological events that impede nutrient supply.
Explanation: The evaluations a Mainly in the oganic responses
