Answer: D. the railroad industry, whose ability to set its own prices was now limited
Explanation:
The Interstate Commerce Act was passed after years of complaints against the Railroad industry which practiced price discrimination and other monopolistic practices that enabled them to make massive profits.
The Act limited their ability to set their own prices and required that their prices be just. This meant that they could no longer charge exorbitant prices. The Railroad industry was therefore opposed to this and lobbied against its passing.
<span> Geographic expansion exposed latent tensions over the morality of slavery and the balance of economic power. It was during the Era of Good Feelings that the political issues arose that would dominate American politics for the next 40 years.</span>
The result would be the destruction of the empire and civilization as they knew back then.
All 4 contributed, but B and D are the most direct causes.