Limiting factors, or things in the environment that can lower the population growth rate, include low food supply and lack of space. When organisms face limiting factors, they show logistic type of growth (S-curve).
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The gene for human insulin is inserted into the gap in the plasmid. this is done through the use of restriction enzymes and ligase to insert and close the plasmid. This plasmid is now genetically modified. The genetically modified plasmid is introduced into a new bacteria or yeast cell. The more the cells divide, the more insulin is produced as bacteria divides into two identical daughter cells like in mitosis.
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