1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Lady_Fox [76]
3 years ago
8

A protein has a molecular mass of 400 kDa when measured by gel filtration. When subjected to SDS-PAGE in the absence of dithioth

reitol (DTT), the protein gives three bands with Mr values of 180,000, 160,000 and 60,000. When SDS-PAGE is performed in the presence of DTT, three bands are again formed, this time with Mr values of 160,000, 90,000 and 60,000. What is the simplest explanation for these results?
Biology
1 answer:
Semmy [17]3 years ago
5 0

Answer:

The protein can be separated by different techniques like SDS PAGE, by the use of urea, and dithiothreitol. The urea and dithiothreitol acts as denaturant that can break the disufide bonds present in the protein.

The total molecular pass of protein is 400,000 Da. In absence of SDS three bands are observed with the mass of  180,000, 160,000 and 60,000. But in presence of dithiothreito These values are observed 160,000, 90,000 and 60,000. This might happen that protein consists of four subunits with 160, 90, 90, and 60 kDa. Without DTT 180,000 subunit is unable to reduce into 90K Da and linked through the disulfide bonds. In presence of DTT the 90,000 units are identical and visible at single lane.

You might be interested in
The genral type of plant food that is produced in the photosynthesis?
____ [38]
B. Carbohydrates
The products of photosynthesis include glucose (a carbohydrate) and oxygen. :D hope this helps
5 0
2 years ago
Read 2 more answers
What group of organisms are responsible for capturing energy within ecosystems
Marina86 [1]

The type of organisms that take energy by eating up other organisms in an ecosystem are called 'CONSUMERS'. Now these consumers are further divided into three major classes:

1. Primary consumers: this type of consumers feed directly from the producers (plants) and they only eat grass, leaves, vegetables, etc. Such animals are also called herbivores. Example: rabbit

2. Secondary consumers: these are the animals that eat up primary consumers (animals that feed only on plants). These animals are called carnivores. Example: snake

3. Tertiary consumers: animals that eat carnivores which eats a herbivore are called tertiary consumers. They can be completely carnivore or omnivore (who feed on animals and plants both). Example: humans (they feed on animals and plants both)

4 0
3 years ago
A theory of evolution that states that a species evolves in spurts of rapid change and then goes through periods of no change is
Aleksandr-060686 [28]
A theory of evolution that states that a species evolves in spurts of rapid change and then goes through periods of no change is known as <span>punctuated equilibrium.</span>
8 0
3 years ago
describe the relationship between the functions of endosymbiotic organelles and their free-living ancestral counterparts
Trava [24]

All organisms arose from a single common ancestor.

An endosymbiont or endobiont is any organism that lives inside the frame or cells of some other organism most often, though no longer continually, in a mutualistic relationship.

Bacterial endosymbionts result in dramatic phenotypes in their arthropod hosts, including cytoplasmic incompatibility, feminization, parthenogenesis, male killing, parasitoid protection, and pathogen blocking.

Endosymbionts, such as Wolbachia, Rickettsia, and Cardinium, are a type of bacteria generally located in arthropod species inclusive of bugs, spiders, crustaceans, and mites, in addition to other invertebrates along with filarial nematodesEndosymbiosis is a form of symbiosis in which the symbiont lives in the body of its host and the symbiont in an endosymbiosis is known as an endosymbiont.

Learn more about endosymbiotic here;-brainly.com/question/1698852

#SPJ4

7 0
9 months ago
Which adaptation makes bipedalism possible?
amid [387]
An adaptation that makes bipedalism possible is the need to get around. If your slithering on the ground, you could get diseases, or get hurt.
7 0
2 years ago
Read 2 more answers
Other questions:
  • Why would it be beneficial for a plant to have more than one type of plant pigment?
    5·1 answer
  • Which statement describes how globes represent Earth’s surface?
    12·2 answers
  • Why does the rate of reaction slow and eventually become constant?
    14·1 answer
  • Damage to the frontal lobes of the brain is a likely result of
    5·2 answers
  • 1 . An individual is a single living thing ? True or False ?
    5·1 answer
  • Binary Fission and Mitosis are similar processes. One way they are different is that:
    7·1 answer
  • What process involves the change of an allele's frequency in a
    8·2 answers
  • Which coated vesicles move materials in a retrograde direction from the ERGIC and Golgi stack backwards toward the ER?
    15·1 answer
  • Who discovered lysosomes<br>​
    6·1 answer
  • What is wild fire as a phrase
    6·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!