It would be considered an opioid.
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
In primary succession, newly exposed or newly formed rock is colonized by living things for the first time. In secondary succession, an area that was previously occupied by living things is disturbed, then re-colonized following the disturbance.
Osmosis is basically the spread of water across a particular absorbent membrane.
<span>The job of these envelope extensions is to increase the bacteria's surface area so they can absorb more nutrients and grow. This is especially important in an adverse environment that lacks a lot of resources.</span>
