They were completely heterophic in nature because it allowed them to absorb different material from the environment as an energy source
In Sanger sequencing, once a modified nucleotide is incorporated into the DNA, there is no free 3'OH group to attach the next nucleotide being added. This phenomenon is useful to sequence a polynucleotide chain.
Dideoxynucleotides (ddNTPs) are nucleotide inhibitors of the DNA polymerase, it is for that reason that they are used in Sanger sequencing.
The ddNTPs are similar to natural deoxynucleotides used by cells during ADN replication; however, ddNTPs lack a hydroxyl (OH) group on the 3’ carbon of the deoxyribose.
In consequence, once a ddNTP is added, DNA synthesis cannot progress, thereby this phenomenon can be used to determine the order of nucleotides during the elongation of a polynucleotide DNA chain.
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