Answer:
b. Forward or reverse primers
Explanation:
Sanger sequencing is a technique of DNA sequencing based on the extension of DNA fragments with variable sizes terminated with dideoxynucleotides at the 3′ end. This technique was developed by Frederick Sanger in 1977. In Sanger sequencing, a short primer is added in order to bind by complementarity to the target DNA region of interest. Subsequently, a DNA polymerase adds nucleotides (A, T, C and G) in the 5'-3' direction. Finally, the extension of the DNA strand is stopped by adding dideoxynucleotides, which are nucleotide analogs (i.e., modified nucleotides) that act as DNA synthesis terminators.
Differences between<span> a </span>physical and chemical change<span> in matter or substances</span>
Answer:
The type of regulation of gene expression that would have the greatest chance of success is Posttranslational control (E).
Explanation:
The defect is found on the structure of the protein, this means that the process of formation of the protein and the codons responsible for each amino acid in the protein is in correct order, this set the transcriptional and translational process aside as being correct.
Therefore, the problem lies in the formation of secondary or tertiary structure of the protein which requires a good number of proteins also, wherein lies tha main problem in the cell membrane protein. Thus the regulation of the posttranslational process and correction of the proteins needed at this stage will give the best chance of success.
